Tập tin:ClpB.gif

Figure 1. The crystal structure of T. thermophilus ClpB.(A) Domain organisation of Hsp104/ClpB. The two AAA domains, although highly conserved, exhibit significant differences and can be classified accordingly as AAA-1 and AAA-2. The middle (M) domain is specific for Hsp104/ClpB and serves for Hsp104/ClpB protein classification. (B) Structure of monomeric T. thermophilus ClpB. The colour code for the individual domains is the same as in (A). AMPPNP is shown in grey. Movements of N and M domains are indicated. (C) Hexameric model of ClpB in the AMPPNP-bound state, based on single-particle reconstitution using cryo-electron microscopy. Height and diameter of the ClpB oligomer is given. The mobile N domains are not visible in the single-particle reconstitution, presumably because they are flexible. M domains were only partially resolved and were placed according to their positioning in the crystal structure [11.].