Establishment of immortal neuronal stem cell line from embryonic day 17 rat brain
Journal of Korean Association of Canver prevention 2003, 8(4): 261-266
Se-Ran Yang, Sung-Dae Cho, Nam-Shik Ahn, Ji-Won Jung, Joon-Suk Park, Nguyen Ba Tiep, Ki-Su Park, In-Sun Hong, Eun-Hye Jo, Min-Su Seo, Byong-Su Yoon, Yong-Soon Lee, Kyung-Sun Kang.
We
have
recently
characterized
Neurosphere-derived
cells
from
embryonic
day
(ED)
17
rat
fetus
brain.
Neural
stem
cells
(NSCs)
have
been
shown
to
reside
in
both
fetal
and
adult
brain
leading
to
formation
of
cell
clusters
termed
"neurospheres"
and
the
neurosphere-derived
cells
can
diffrentiate
into
neurons,
oligodendrocytes
and
astrocytes.
In
this
study,
we
reported
the
establishment
of
neuronal
stem
cell
line
using
SV40
large
T
antigen.
The
results
show
that
the
cells
were
acquired
extended
life
span
and
constant
increase
at
early
(about
30
cumulative
population
doubling
level
(cpdl)),
middle
(60
cpdl),
and
late
(100
cpdl)
passages.
To
characterize
the
established
immortal
cell,
immunofluorescent
staining
was
performed
using
GFAP
antibody
for
astrocyte,
NSE
for
neuron,
and
Nestin
for
neuronal
stem
cells.
These
results
showed
that
the
established
immortal
cell
line
expressed
nestin.
These
results
suggest
that
the
cell
lines
might
be
a
neuronal
stem
cells,
and
the
stem
cells
might
be
also
major
target
cells
for
the
carcinogenesis.
Key words: Immortalization, stem cells, SV40 Large T-antigen, Nestin, Neurosphere-derived cells
